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Updated: Jun 4, 2025

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Carl Prévost-Tremblay1, Achille Vigneault2, Dominic Lauzon3

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|December 19, 2024
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Summary
This summary is machine-generated.

Researchers developed a DNA switch demonstrating programmable control over molecular switch kinetics. This work clarifies the advantages of induced fit (IF) and conformational selection (CS) mechanisms for designing artificial molecular systems.

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Area of Science:

  • Molecular biology
  • Chemical engineering
  • Nanotechnology

Background:

  • Biomolecular switches control cellular functions via chemical signals.
  • Two main mechanisms, induced fit (IF) and conformational selection (CS), govern switch kinetics.
  • The kinetic and evolutionary advantages of IF and CS remain poorly understood.

Purpose of the Study:

  • To create a modular DNA switch controllable by both IF and CS mechanisms.
  • To characterize the thermodynamic and kinetic parameters of these mechanisms.
  • To demonstrate programmable control over molecular switch kinetics.

Main Methods:

  • Designed and synthesized a modular DNA-based molecular switch.
  • Investigated switch behavior under conditions favoring IF and CS.
  • Quantified thermodynamic and kinetic parameters.
  • Engineered a drug delivery vessel using the DNA switch.

Main Results:

  • The fastest switch activation occurred via the induced fit (IF) mechanism.
  • Conformational selection (CS) allowed for multi-order magnitude programming of activation rates.
  • A drug delivery vessel demonstrated programmable drug release over >1000-fold time scales.

Conclusions:

  • Developed a programmable strategy for optimizing molecular switch kinetics.
  • Demonstrated the utility of IF and CS mechanisms in designing artificial molecular systems.
  • Provided insights into the evolutionary advantages of IF and CS in biomolecular switches.