As, Cd, Hg, and Pb Biological Concentrations and Anthropometry in Slovak Adolescents

Anthropometry provides a non-invasive technique for evaluating growth and obesity and serves as an indicator of health status. This cross-sectional study aims to investigate the association of internal arsenic (As), cadmium (Cd), total mercury (THg), methylmercury (MeHg), and lead (Pb) exposure with anthropometric parameters, including obesity, in adolescents. Participants (N = 320) were children aged 10-14 years (mean 11.8 years) from eastern Slovakia, at an early stage of adolescence characterized by growth acceleration. Metal concentrations in blood and urine were measured by ICP-MS (for As, Cd, and Pb), GC/ICP-MS (for MeHg) and amalgamation technique AAS (for THg). Median concentrations of the studied elements in whole blood (Cd: 0.16, Pb: 10.6, THg: 0.25, MeHg: 0.11 µg/L) and urine (Cd: 0.25, Pb: 0.73, As: 3.38 µg/g creatinine) were relatively low in our study group. The results showed that blood Cd and Pb concentrations were inversely associated with several anthropometric parameters (body weight, BMI, body fat percentage, chest circumference, and waist circumference) in both boys and girls. Conversely, blood THg concentration was positively associated with these parameters in boys. A positive relationship was also observed between blood MeHg concentration and height in boys, while negative associations between blood Cd and Pb concentrations and height were significant only in girls. No associations were found between metal concentrations (As, Cd, Pb) in urine and parameters of physical growth or obesity. This study demonstrates that even low-level exposure to Cd, Pb, and Hg can influence growth and obesity indicators in adolescents, with distinct sex-specific patterns, highlighting the need for ongoing monitoring and protection against environmental metal exposure.