Multi-responsive biosensor prepared based on MXene and PDEA-HRP binary architecture films for H₂O₂ detection and...

Area of Science:

• Electrochemistry
• Biosensors
• Nanomaterials

Background:

• Quantitative detection of hydrogen peroxide (H₂O₂) is vital for disease prevention.
• Electrochemical biosensors offer a sensitive platform for H₂O₂ detection.

Purpose of the Study:

• To construct a novel electrochemical biosensor for sensitive H₂O₂ detection.
• To explore the application of the biosensor in constructing biomolecular logic gate systems.

Main Methods:

• Step-by-step modification of a pyrolytic graphite (PG) electrode.
• Incorporation of MXene nanomaterials for enhanced conductivity and biocompatibility.
• Coating with poly(N,N-dimethyl acrylamide)-horseradish peroxidase (PDEA-HRP) hydrogel for H₂O₂ detection and logic gate functionality.

Main Results:

• The PDEA-HRP/MXene/PG biosensor demonstrated high sensitivity and a wide linear detection range (0.04 mM–1.80 mM) for H₂O₂.
• A low detection limit of 1.08 × 10⁻³ mM (S/N = 3) was achieved with good repeatability.
• The biosensor was successfully applied to detect H₂O₂ in actual samples.
• Construction of biomolecular electrocatalytic logic gate systems, including a 5-input/5-output network, a 2-to-4 decoder, and ternary AND gates.

Conclusions:

• The developed PDEA-HRP/MXene/PG biosensor is a sensitive and reliable tool for H₂O₂ detection.
• The unique properties of MXene and PDEA-HRP hydrogel enable advanced applications in biomolecular logic systems.
• This work provides a promising platform for both disease diagnostics and complex bio-signal processing.