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Exposure to oxygenated polycyclic aromatic hydrocarbons and endocrine dysfunction: Multi-level study based on hormone
Ying Ren1, Yue Wang2, Yang Wang1
1Shanxi Key Laboratory of Coal-based Emerging Pollutant Identification and Risk Control, Research Center of Environment and Health, College of Environment and Resource, Shanxi University, Taiyuan, Shanxi 030006, PR China.
Journal of Hazardous Materials
|December 19, 2024
Summary
Oxygenated polycyclic aromatic hydrocarbons (OPAHs) can disrupt endocrine systems. This study found that 1,2-benzanthraquinone (BAQ) shows strong hormonal activity and causes developmental toxicity in zebrafish larvae.
Area of Science:
- Environmental Science
- Toxicology
- Endocrinology
Background:
- Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are emerging contaminants with high toxicity.
- OPAHs' endocrine-disrupting potential is debated, with controversial reports.
- Understanding OPAHs' interaction with hormone receptors is crucial for risk assessment.
Purpose of the Study:
- To investigate the endocrine-disrupting effects of typical OPAHs.
- To identify specific OPAHs with significant hormonal activity.
- To elucidate the mechanisms of OPAH-receptor interactions and their developmental toxicity.
Main Methods:
- Dual luciferase reporter gene assay and molecular docking to assess OPAH-hormone receptor interactions.
- Development of a weighted scoring system for OPAH hormonal effect screening.
- In vivo zebrafish larvae exposure model to evaluate developmental toxicity.
- Construction of androgen response element-enhanced green fluorescent protein (ARE-EGFP) zebrafish embryos.
Main Results:
- Five typical OPAHs showed agonistic activity towards hormone receptors (ERα/AR/GRα/TRβ).
- Hydrogen bonding and hydrophobic interactions mediate OPAH-receptor binding.
- 1,2-benzanthraquinone (BAQ) exhibited the strongest hormonal effects, while anthraquinone (AQ) showed the weakest.
- BAQ induced developmental toxicity in zebrafish, including altered androgen receptor expression and key hormone synthesis genes, pericardial edema, and reduced body length.
- BAQ demonstrated androgenic potency in ARE-EGFP zebrafish embryos, unlike AQ.
Conclusions:
- OPAHs, particularly BAQ, possess significant endocrine-disrupting capabilities.
- BAQ's hormonal activity is linked to developmental toxicity in aquatic organisms.
- These findings underscore the importance of considering OPAHs' endocrine-disrupting effects in environmental risk management.
Keywords:
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