New insights into autochthonous fungal bioaugmentation mechanisms for recalcitrant petroleum hydrocarbon components...

Autochthonous fungal bioaugmentation (AFB) is a promising strategy for the microbial remediation of petroleum hydrocarbon (PH)-contaminated soils. However, the mechanisms underlying AFB, particularly for degrading recalcitrant PH components, are not fully understood. This study employed stable isotope probing (SIP) and high-throughput sequencing to investigate the AFB mechanisms of two hydrocarbon-degrading fungi, Fusarium solani LJD-11 and Aspergillus fumigatus LJD-29, focusing on three challenging PH components: n-Hexadecane (n-Hex), Benzo[a]pyrene (BaP), and Dibenzothiophene (DBT). Our findings indicate that both fungal strains significantly enhanced pollutant removal rates, with combined application yielding optimal results. AFB treatment reduced the microbial diversity index and altered the soil microbial community, especially affecting fungal populations. A significant correlation between the microbial diversity index and degradation efficiency suggests that greater diversity enhances pollutant removal. SIP analysis showed that LJD-11 and LJD-29 could directly assimilate n-Hex and DBT, but not BaP. Correlation analyses between functional microorganisms and other biological indicators suggest that the removal of pollutants is also attributable to indigenous functional bacteria. Additionally, non-inoculated functional fungi present in the soil play a crucial role in BaP degradation. These findings reveal distinct degradation pathways for the three pollutants. The addition of carrier substrate reduced the complexity of the network, while AFB treatment restored it. In addition, the combined fungal treatment resulted in higher network parameters, leading to a more complex and stable network structure. These results provide insights into the mechanisms of AFB for degrading recalcitrant PH components, underscoring its potential for in situ bioremediation of petroleum-contaminated soils.