Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intestinal membrane lipid composition and fluidity during development in the rat.

S M Schwarz, B Hostetler, S Ling

    The American Journal of Physiology
    |February 1, 1985
    PubMed
    Summary

    Young rats have more fluid small intestinal microvillus membranes (MVM) than mature rats, with differences in lipid composition and fluidity between jejunum and ileum. These age-dependent and regional variations impact MVM biophysical properties.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Age-Specific Socioeconomic Inequalities in Treatment in Patients with Stage III Colon Cancer in England 2012-2016: A Population-Based Study with Mediation Analysis.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2025
    Same author

    Generating fair, reliable, and accurate neuropsychological test norms for people with HIV in a low- or middle-income country.

    Journal of neurovirology·2024
    Same author

    Incidence and mortality of sepsis in Hong Kong between 2009 and 2018 based on electronic health records: abridged secondary publication.

    Hong Kong medical journal = Xianggang yi xue za zhi·2024
    Same author

    Diabetes in adults with intellectual disability: prevalence and associated demographic, lifestyle, independence and health factors.

    Journal of intellectual disability research : JIDR·2020
    Same author

    Is vertebral body stenting in combination with CaP cement superior to kyphoplasty?

    European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2018
    Same author

    Dunye Guanxinning Improves Acute Myocardial Ischemia-Reperfusion Injury by Inhibiting Neutrophil Infiltration and Caspase-1 Activity.

    Mediators of inflammation·2018

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Physiology

    Background:

    • Microvillus membranes (MVM) are crucial for nutrient absorption in the small intestine.
    • MVM lipid composition and fluidity are vital for intestinal function and can change with age and diet.

    Purpose of the Study:

    • To characterize the lipid composition and membrane fluidity of rat jejunal and ileal MVM in suckling and mature animals.
    • To investigate age-dependent and regional differences in MVM biophysical properties.

    Main Methods:

    • Fluorescence polarization using 1,6-diphenyl-1,3,5-hexatriene (DPH) as a probe to estimate membrane fluidity.
    • Analysis of MVM lipid composition, including total lipid, cholesterol, phospholipid, and fatty acid profiles.
    • Determination of thermotropic lipid-phase transitions using Arrhenius plots.

    Related Experiment Videos

    Main Results:

    • MVM from suckling rats exhibited increased fluidity compared to mature rats, indicating a more disordered molecular environment.
    • Ileal MVM were consistently less fluid than jejunal MVM in both age groups.
    • Suckling animals had higher MVM lipid, cholesterol, and phospholipid content per milligram of protein.
    • A thermotropic lipid-phase transition was observed at 23°C in mature rat MVM, but not in suckling rats.

    Conclusions:

    • Small intestinal MVM display significant age-dependent and regional (proximal-distal) variations in lipid composition and biophysical properties.
    • Protein-lipid interactions are key determinants of MVM fluidity.
    • These findings highlight developmental changes in the intestinal barrier function and nutrient absorption efficiency.