Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Types of RNA01:23

Types of RNA

63.2K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.2K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

20.8K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
20.8K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

1
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
1
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.6K
Riboswitches01:56

Riboswitches

8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

28.7K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
28.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of Antibiofilm Molecules from Bovine Coagulase-Negative Staphylococci.

PloS one·2026
Same author

Regulation of iron ion homeostasis in <i>Staphylococcus aureus</i> and its impact on physiology and virulence.

mBio·2026
Same author

<i>Staphylococcus aureus</i> can degrade exogenous fatty acids through β-oxidation.

mBio·2026
Same author

An anti-adhesive compound modulating the production of Staphylococcus aureus cell wall-anchored proteins.

Nature communications·2026
Same author

Insights into bacterial stress adaptation, host interactions, and drug resistance: key findings from the fall 2025 ASM Theobald Smith Society meeting.

mSphere·2026
Same author

Genetic basis of cefiderocol resistance in <i>Acinetobacter baumannii</i>: insights from functional genomics and clinical isolates.

Microbiology spectrum·2026

Related Experiment Video

Updated: Jun 4, 2025

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.8K

The Staphylococcus aureus non-coding RNA IsrR regulates TCA cycle activity and virulence.

Gustavo Rios-Delgado1, Aubrey K G McReynolds2, Emma A Pagella2

  • 1Department of Biochemistry and Microbiology, Rutgers, the State University of New Jersey, 76 Lipman Dr, New Brunswick, NJ 08901, USA.

Nucleic Acids Research
|December 20, 2024
PubMed
Summary

Staphylococcus aureus uses Fur to regulate iron uptake. A regulatory RNA, IsrR, was found to enhance iron acquisition and bacterial infection, independent of Fur.

More Related Videos

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

1.6K
Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

406

Related Experiment Videos

Last Updated: Jun 4, 2025

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.8K
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

1.6K
Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

406

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Pathogenesis

Background:

  • Staphylococcus aureus adapts to low iron conditions in hosts.
  • The ferric uptake transcriptional regulator (Fur) controls iron homeostasis.
  • Iron availability impacts bacterial growth and virulence.

Purpose of the Study:

  • Investigate the role of Fur and regulatory RNA in Staphylococcus aureus iron metabolism.
  • Identify mechanisms of iron acquisition and their impact on virulence.
  • Characterize the interaction between IsrR and its target transcripts.

Main Methods:

  • Utilized a suppressor screen to identify mutations affecting bacterial growth.
  • Performed gene expression analysis (acnA, sdhC, mqo, citZ, citM).
  • Conducted in vitro binding assays and in vivo infection models (murine skin, pneumonia).

Main Results:

  • A Δfur mutant showed reduced acnA expression and impaired growth.
  • Decreased IsrR transcription restored acnA expression and growth.
  • IsrR directly binds acnA and other metabolic transcripts, promoting iron uptake.
  • Both Fur and IsrR contribute to Staphylococcus aureus infection.

Conclusions:

  • IsrR plays a significant role in Staphylococcus aureus iron acquisition and virulence.
  • IsrR acts independently of Fur to modulate iron-dependent gene expression.
  • Targeting IsrR could be a strategy to combat Staphylococcus aureus infections.