Related Experiment Video
Updated: Jun 4, 2025
09:27
High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
Published on: May 10, 2016
8.1K
COBRA-LIKE 9 modulates cotton cell wall development via regulating cellulose deposition
Qingfei He1,2, Yujia Yu1,2, Zhiguang Qin1,2,3
1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Plant Physiology
|December 20, 2024
Summary
Cotton COBRA-LIKE 9 (GhCOBL9) protein is crucial for plant cell wall development. It enhances cellulose content, leading to stronger cotton fibers and revealing new insights into COBL protein functions.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Plant cell walls are vital for growth and adaptation, with cellulose being a key component.
- The COBRA-LIKE (COBL) protein family influences cellulose microfibril deposition and orientation.
- Understanding cellulose assembly mechanisms is crucial for plant science.
Purpose of the Study:
- To investigate the role of GhCOBL9 in cotton fiber cell wall development.
- To elucidate the molecular mechanisms underlying GhCOBL9 function in cellulose deposition.
Main Methods:
- Gene expression analysis (RT-qPCR).
- Overexpression and RNA interference (RNAi) in cotton.
- Subcellular localization and binding assays.
- Transcriptomic analysis.
- Protein-protein interaction assays (Yeast two-hybrid).
- Promoter analysis.
Main Results:
- GhCOBL9 expression correlates with cotton fiber secondary wall thickening.
- GhCOBL9 overexpression increases cellulose content and fiber strength, while RNAi shows opposite effects.
- GhCOBL9 targets the cell wall and binds crystalline cellulose.
- GhCOBL9 interacts with GhFLA9 and is regulated by GhMYB46-5.
Conclusions:
- GhCOBL9 is essential for cotton fiber cell wall development by modulating cellulose deposition and orientation.
- GhCOBL9 functions by directly binding to crystalline cellulose.
- GhCOBL9's activity is influenced by interacting proteins and transcription factors, providing a comprehensive view of its regulatory network.
Related Concept Videos
01:02Role of Microtubules in Cell Wall Deposition
2.3K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.3K
01:15Cellulose and Pectic Polysaccharides
3.5K
Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
As a cell matures, its cell wall specializes according to its type. For example, the...
3.5K
02:43Plant Cell Wall
54.7K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
54.7K
01:27C4 Pathway and CAM
45.2K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.2K
01:14Cell Adhesion in Plants
2.6K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
2.6K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K