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Improving Infrared Spectroscopy Characterization of Soil Organic Matter with Spectral Subtractions

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Published on: January 10, 2019

Probing Mineral-Organic Interfaces in Soils and Sediments Using Optical Photothermal Infrared Microscopy.

Floriane Jamoteau¹, Mustafa Kansiz², Miriam Unger²

¹Institute of Earth Surface Dynamics, University of Lausanne, Lausanne 1015 CH, Switzerland.

Environmental Science & Technology

|December 20, 2024

Summary

Optical photothermal infrared (O-PTIR) microscopy offers submicron-scale analysis of complex soil and sediment interactions. This technique non-destructively characterizes mineral-organic matter and microbe associations, crucial for understanding environmental dynamics.

Keywords:

IR spectro-microscopy O-PTIR biofilms microstructures mid infrared mineral-organic interfaces minerals sorption

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In situ FTIR Spectroscopy as a Tool for Investigation of Gas/Solid Interaction: Water-Enhanced CO2 Adsorption in UiO-66 Metal-Organic Framework

Published on: February 1, 2020

Area of Science:

Environmental Science
Analytical Chemistry
Microscopy

Background:

Soil and sediment dynamics are governed by intricate microbe-mineral-organic matter interactions.
Analyzing these interactions at relevant scales and over time is analytically challenging.
Existing methods lack the necessary non-destructive, real-time, and high-spatial-resolution capabilities.

Purpose of the Study:

To demonstrate the utility of optical photothermal infrared (O-PTIR) microscopy for submicron-scale characterization of mineral-organic microstructures.
To provide analytical recommendations for using O-PTIR microscopy in environmental samples.
To assess O-PTIR's potential for non-destructive, time-resolved analysis of soil and sediment interactions.

Main Methods:

Submicron-scale characterization using optical photothermal infrared (O-PTIR) microscopy.
Comparison of O-PTIR spectra with conventional infrared techniques for mineral and organic reference compounds.
Evaluation of O-PTIR sensitivity and resolution for mineral-bound and unbound organics.
Determination of optimal laser power for artifact-free analysis using damage thresholds.

Main Results:

O-PTIR microscopy achieved submicron (<500 nm) resolution for complex mineral-organic microstructures.
O-PTIR demonstrated comparable spectral quality and sensitivity to conventional infrared techniques.
O-PTIR exhibited enhanced sensitivity towards organic matter, particularly mineral-bound organics, due to photothermal effects.
Best practices for artifact-free O-PTIR analysis were established.

Conclusions:

O-PTIR microscopy is a powerful tool for non-destructive, submicron-scale characterization of mineral-organic matter interactions.
The technique holds significant potential for time-resolved studies of dynamic processes in soils and sediments.
O-PTIR enables detailed analysis of complex environmental matrices, advancing our understanding of biogeochemical cycles.