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Updated: Jun 4, 2025

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
Published on: October 30, 2014
Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS
Joan Antoni Soler1, Anupam Singh1,2, Marino Zerial1
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Abstract:
Fluorescence correlation spectroscopy (FCS) enables the measurement of fluctuations at fast timescales (typically few nanoseconds) and with high spatial resolution (tens of nanometers). This single-molecule measurement has been used to characterize single-molecule transport and flexibility of polymers and biomolecules such as DNA and RNA. Here, we apply this technique as dual-color fluorescence cross-correlation spectroscopy (dcFCCS) to identify the motor function of the tethering protein EEA1 and the small GTPase Rab5 by probing the flexibility changes through end-monomer fluctuations.
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