Related Experiment Video

Updated: Jun 4, 2025

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
08:16

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors

Published on: July 27, 2022

1.9K

Tracking Single Kinesin in Live Cells Using MINFLUX

Takahiro Deguchi1, Nikolay Arkadievich Sergeev1,2,3, Jonas Ries4,5,6,7

  • 1European Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|December 20, 2024
PubMed

Keywords:
Conformational dynamicsFluorescence microscopyKinesinMINFLUXMotor proteinSingle-molecule trackingSuper-resolution

More Related Videos

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

3.4K
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells10:46

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Published on: February 2, 2022

2.5K

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
5.8K