JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Trop-2 and Ephrin B2 expression in urothelial carcinoma with divergent differentiation and aggressive urothelial carcinoma subtypes

  • 0Emory University School of Medicine, Atlanta, GA, US.
American Journal of Clinical Pathology +

|

December 20, 2024

|

December 20, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Trop-2 and ephrin B2 show potential as therapeutic targets in aggressive urothelial carcinomas (UCs) with divergent differentiation. These findings offer new hope for patients with limited treatment options, especially in small cell carcinoma.

Area Of Science

  • Oncology
  • Urothelial Carcinomas
  • Molecular Pathology

Background

  • Urothelial carcinomas (UCs) are a heterogeneous cancer group.
  • Specific histopathologic features correlate with poor prognosis and limited therapeutic strategies.
  • Emerging treatments necessitate identification of novel therapeutic targets.

Purpose Of The Study

  • To investigate the expression patterns of Trop-2 and ephrin B2.
  • To analyze these markers in UC subtypes with aggressive histology and/or divergent differentiation (SH/DD).

Main Methods

  • Retrospective analysis of 113 UC samples (2011-2021).
  • Immunohistochemical staining for Trop-2 and ephrin B2.
  • Expression quantified by H-score (moderate or strong staining).

Main Results

  • Trop-2 expression was highest in squamous cell carcinoma, UC with squamous differentiation, adenocarcinoma, UC with glandular differentiation, and plasmacytoid subtype.
  • Ephrin B2 expression was highest in adenocarcinoma, UC with glandular differentiation, and small cell carcinoma.

Conclusions

  • Trop-2 and ephrin B2 expression suggests potential therapeutic avenues for SH/DD UCs.
  • These markers may be particularly relevant for small cell carcinoma, a subtype with few identified targets.
  • Further clinical trials are recommended to evaluate novel treatment efficacy.

Keywords:

Related Concept Videos

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal 01:22

2.2K

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Cadherins in Tissue Organization 01:19

2.9K

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...

Mitogens and the Cell Cycle 02:38

6.4K

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...