Comprehensive performance of a new-type hybrid membrane bioreactor applied to mainstream anammox process

The new-type submerged granular sludge membrane bioreactor (S-GSMBR) was constructed by installing a membrane module inside an upflow anaerobic sludge blanket. S-GSMBR achieved the fast start-up (47 d) and long-term stable operation (133 d) of mainstream Anammox process as well as the effective control of membrane fouling. The maximum nitrogen removal rate and efficiency were 0.52 kg N m^-3 d^-1 and 88.32%. The flow field analysis indicated that the turbulent effects were found around the membrane module and benefited for alleviating the membrane fouling at the special axial flow velocity at the central section of the membrane module (4.89 × 10^-7 m s^-1 to 1.30 × 10^-6 m s^-1). On day 133, S-GSMBR achieved sludge granulation with 68.63% of particles larger than 200 μm. Meanwhile, Candidatus Brocadia and Candidatus Kuenenia were typical Anammox bacteria in mature Anammox granular sludge. The relative abundance of Candidatus Brocadia and Candidatus Kuenenia was 21.22% and 0.36%, indicating that Candidatus Brocadia occupied the main niche under mainstream Anammox conditions. The feasibility of application of S-GSMBR in mainstream Anammox process was confirmed and moreover S-GSMBR was proven to be a robust mainstream Anammox reactor. This study lays the important theoretical foundation and technical support for the engineering application of the mainstream Anammox process.