Forest management impacts on soil phosphorus cycling: Insights from metagenomics in Moso bamboo plantations

Bamboo forests are crucial ecosystems and provide essential ecological and economic services in both tropical and subtropical regions. Soil phosphorus (P), a vital nutrient for plant growth, is fundamental to the productivity and health of bamboo forests. However, the microbial mechanisms through which management practices affect soil P processes in bamboo forests remain poorly understood. This study employed metagenomics to examine alterations in microbial P cycling in Moso bamboo plantations under three distinct management conditions. The results revealed that intensive management (M2, annual fertilization, selective harvesting, and understory vegetation removal) significantly increased soil inorganic P (Pi) by 61.76% and 87.39% compared to extensive management (M1, selective bamboo trunk and shoot harvesting every two years) and non-management (M0), respectively, while decreasing soil organic P (Po) by 50.41% and 41.05%. Forest management significantly altered the bacterial communities: Firmicutes, WPS-2, and Acidobacteriales were represented in M2, Xanthobacteraceae in M1, and Chloroflexi AD3, Acidothermus, and Subgroup_2 in M0. M2 significantly increased the community-level habitat niche breadth and weakened the deterministic process of bacterial community assembly relative to M1 and M0 (p ≤ 0.05). Furthermore, functional metagenomics showed that the total abundance of genes related to Po mineralization, P transportation, and P regulation was significantly lower (p ≤ 0.05) in M2 than in M0 and M1. pstA, pstB, and pstC were more abundant in M2 (p ≤ 0.05), whereas phnN, phnI, phnG, phoA, phoD, phnC, phnD, and phnE were more abundant in M1 (p ≤ 0.05), and phnF was significantly abundant in M0 (p ≤ 0.05). A partial least squares path model indicated that soil bacterial community and P cycling genes had direct effects on Pi and Po, respectively. These findings enhance our understanding of the links between forest management practices and P cycling, providing insights for improving soil functionality and nutrient balance.