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Structural identification of platelet activating factor in psoriatic scale.
Biochemical and Biophysical Research Communications
|January 16, 1985
Summary
Researchers isolated platelet-activating factor (PAF) from psoriatic scales. This study identified specific alkyl chain lengths, C16 and C18, in the recovered PAF, offering insights into its role in psoriasis.
Area of Science:
- Dermatology
- Biochemistry
- Lipidomics
Background:
- Psoriasis is a chronic inflammatory skin condition.
- Platelet-activating factor (PAF) is a potent lipid mediator implicated in inflammatory processes.
Purpose of the Study:
- To isolate and characterize platelet-activating factor (PAF) from psoriatic scales.
- To determine the alkyl chain composition of PAF in psoriatic lesions.
Main Methods:
- Isolation of PAF from psoriatic scales using Bligh and Dyer method.
- Separation and identification via thin-layer chromatography (TLC), phospholipase C treatment, reversed-phase high-performance liquid chromatography (RP-HPLC), and capillary gas chromatography-mass spectrometry (GC-MS).
- Assay of platelet aggregation activity using washed guinea-pig platelets.
Main Results:
- Nanogram quantities of PAF were successfully recovered from 100 mg of psoriatic scale.
- The recovered PAF predominantly featured C16 and C18 alkyl substituents in a 3:1 ratio (C16:C18).
Conclusions:
- Platelet-activating factor (PAF) is present in psoriatic scales.
- The specific alkyl chain lengths (C16 and C18) of PAF in psoriasis may influence its biological activity and role in the disease pathogenesis.