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Immunoreactive fibroblast growth factor in cells of peritoneal exudate suggests its identity with macrophage-derived
Abstract:
Peritoneal exudate cells were collected from thioglycollate stimulated mice, extracted an examined for the presence of immunoreactive and bioactive fibroblast growth factor (FGF). The crude extract stimulated in a dose dependent fashion the proliferation of vascular endothelial cells derived from the bovine aortic arch. The extract also showed a parallel and dose-dependent inhibition of binding in a highly specific radioimmunoassay for FGF. The immunoreactive FGF (ir-FGF) contained in the extract was retained on a heparin-sepharose affinity column as is characteristic of pituitary FGF. Reverse-phase HPLC of the macrophage-derived material reveals one biologically active form of FGF which coelutes with the major form of immunoreactivity. The results demonstrate the presence of FGF in these cells and suggest that at least one of the hitherto unidentified mitotic activities in these extracts is due to a mitogen indistinguishable from FGF.
Insights
This study found fibroblast growth factor (FGF) in mouse peritoneal cells. This FGF stimulates vascular endothelial cell proliferation, indicating its role in cell growth and repair.
Area of Science:
- Cell Biology
- Molecular Biology
- Immunology
Background:
- Peritoneal exudate cells (PECs) are a source of bioactive molecules.
- Fibroblast growth factor (FGF) is a key regulator of cell proliferation and angiogenesis.
- The presence and function of FGF in PECs remain largely uncharacterized.
Purpose of the Study:
- To investigate the presence and characteristics of fibroblast growth factor (FGF) in peritoneal exudate cells (PECs).
- To determine the bioactivity and immunoreactivity of FGF derived from PECs.
- To identify the specific form of FGF present in these cells.
Main Methods:
- Collection and extraction of peritoneal exudate cells from thioglycollate-stimulated mice.
- Assay of cell proliferation using bovine aortic arch endothelial cells.
- Radioimmunoassay (RIA) for FGF binding inhibition.
- Heparin-sepharose affinity chromatography.
- Reverse-phase High-Performance Liquid Chromatography (RP-HPLC).
Main Results:
- Crude PEC extract stimulated vascular endothelial cell proliferation in a dose-dependent manner.
- The extract demonstrated dose-dependent inhibition of FGF binding in a specific radioimmunoassay.
- Immunoreactive FGF (ir-FGF) from PECs bound to heparin-sepharose, similar to pituitary FGF.
- RP-HPLC identified a single biologically active FGF form co-eluting with the major immunoreactive form.
Conclusions:
- Peritoneal exudate cells contain immunoreactive and bioactive fibroblast growth factor (FGF).
- The FGF present in PECs is biologically active and indistinguishable from known FGF forms.
- These findings suggest FGF contributes to the previously unidentified mitogenic activities in PEC extracts.