A non-fluorescent complex of chlorophyll a with plastocyanin

A complex between chlorophyll a and plastocyanin has been prepared by dialysis of mixtures of chlorophyll in Triton X-100 micellar solution with the protein. The complex appears to contain no more than one chlorophyll per plastocyanin molecule, and is non-fluorescent, whether the protein is in the oxidized or reduced state. The lack of fluorescence suggests that the chlorophyll is adsorbed very close to the Cu center.