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[Recombinant VLP Vaccines Synthesized in Plant Expression Systems: Current Updates and Prospects].

S M Rozov1,2, E V Deineko1,3

  • 1Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia.

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Summary

New virus-like particle (VLP) vaccines utilize recombinant proteins for strong immune responses. Plant-based expression systems offer a promising method for producing these VLPs efficiently.

Keywords:
VLP vaccineplant expression systemsrecombinant proteinsstable expressiontransient expressionvirus-like particles

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Area of Science:

  • Biotechnology
  • Vaccinology
  • Molecular Biology

Background:

  • Recombinant protein vaccines, particularly virus-like particles (VLPs) and nanoparticles, represent a significant advancement in modern biotechnology.
  • VLPs, due to their size and repetitive antigenic determinants, elicit robust humoral and cellular immunity.

Purpose of the Study:

  • This review examines various VLP types and their expression systems.
  • The focus is on plant-based expression systems for VLP biosynthesis.

Main Methods:

  • Exploration of stable and transient expression in plant nuclear and chloroplast genomes.
  • Discussion of strategies to enhance recombinant protein yield, such as chloroplast transit peptides.
  • Consideration of protein body accumulation using specific signal sequences for VLP vaccine components.

Main Results:

  • Plant expression systems provide a viable platform for VLP production.
  • Yield enhancement strategies are crucial for efficient VLP-based vaccine development.
  • Targeted accumulation in protein bodies can facilitate VLP component storage.

Conclusions:

  • Plant expression systems are a promising avenue for developing next-generation VLP vaccines.
  • Optimizing expression and accumulation strategies is key to successful VLP vaccine manufacturing.