Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

728
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
728
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.3K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.3K
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

334
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
334

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent advances in microresonators and supporting instrumentation for electron paramagnetic resonance spectroscopy.

The Review of scientific instruments·2022
Same author

Overhauser dynamic nuclear polarization (ODNP)-enhanced two-dimensional proton NMR spectroscopy at low magnetic fields.

Magnetic resonance (Gottingen, Germany)·2022
Same author

Compact, tunable polarization transforming reflector for quasi-optical devices used in terahertz science.

The Review of scientific instruments·2022
Same author

The Role of Backbone Polarity on Aggregation and Conduction of Ions in Polymer Electrolytes.

Journal of the American Chemical Society·2020
Same author

High-resolution Overhauser dynamic nuclear polarization enhanced proton NMR spectroscopy at low magnetic fields.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2020
Same author

Tau-Cofactor Complexes as Building Blocks of Tau Fibrils.

Frontiers in neuroscience·2020
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2025

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
06:42

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Published on: September 1, 2020

3.4K

Cryogenic sample eject system for electron paramagnetic resonance spectrometers.

Karl Rieger1, Joshua Hoy1, Timothy J Keller1

  • 1Bridge12 Magnetic Resonance, 11 Michigan Drive, Natick, MA 01760, USA.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|December 21, 2024
PubMed
Summary
This summary is machine-generated.

A new automated system for cryogenic sample insertion and ejection in Electron Paramagnetic Resonance (EPR) spectroscopy enables reliable low-temperature experiments. This system improves sample quality, yielding better glass matrices and more accurate measurements at temperatures as low as 10 K.

Keywords:
AutomationDEEREPR SpectroscopyINSTRUMENTATIONPELDOR

More Related Videos

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

17.6K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

269

Related Experiment Videos

Last Updated: Jun 4, 2025

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
06:42

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Published on: September 1, 2020

3.4K
Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

17.6K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

269

Area of Science:

  • Cryogenics
  • Spectroscopy
  • Materials Science

Background:

  • Low-temperature Electron Paramagnetic Resonance (EPR) spectroscopy requires precise sample handling.
  • Conventional methods for sample manipulation at cryogenic temperatures can be challenging and may affect sample integrity.
  • Optimizing sample environment is crucial for accurate EPR measurements, particularly for studying glass properties.

Purpose of the Study:

  • To develop and validate a fully automated cryogenic sample insertion and ejection system for low-temperature EPR.
  • To assess the system's performance at temperatures down to 10 K.
  • To investigate the impact of the automated system on the glass properties of a TEMPO sample.

Main Methods:

  • Implementation of an automated cryogenic sample insertion/ejection system on a conventional EPR spectrometer.
  • Measurement of electron phase memory time (Tm) to evaluate glass properties.
  • Determination of effective spin concentration using PELDOR/DEER background traces.
  • Comparison of results from the automated system versus manually flash-frozen samples.

Main Results:

  • Reliable sample insertion and ejection demonstrated at temperatures as low as 10 K.
  • The automated system consistently produced a superior glass matrix compared to manual freezing.
  • Longer electron phase memory times (Tm) were observed with the automated system.
  • Lower effective spin concentrations were determined using the automated system.

Conclusions:

  • The developed automated cryogenic sample handling system is effective for low-temperature EPR.
  • The system enhances sample quality, leading to improved glass matrices and more reliable experimental data.
  • This automation offers a significant advantage for cryogenic EPR studies requiring precise sample preparation and handling.