Related Experiment Video
Updated: Jun 4, 2025

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
Chlorine and UV combination sequence: Effects on antibiotic resistance control and health risks of ARGs
Jingyi Zhang1, Weiguang Li1, Xinming Guo1
1State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China; School of Environment, Harbin Institute of Technology, Harbin, 150090, China.
Abstract:
The effective control of antibiotic resistance in aquatic environments is urgent. The combined chlorine and UV processes (Cl2-UV, UV/Cl2, and UV-Cl2) are considered potential control processes for controlling antibiotic resistance. This study compared the effectiveness of these three processes in real water bodies and the potential health risks associated with antibiotic resistance genes (ARGs) after treatments. The removal of various antibiotic-resistant bacteria (ARB) and ARGs by the combined processes was analysed. The UV/Cl2 process was less effective than the others in inactivating β-lactam-resistant bacteria (BRB) and sulfamethoxazole-resistant bacteria (SRB), which are more challenging to remove, though its performance might improve with increased UV fluence. Nevertheless, the UV/Cl2 process showed an advantage in removing ARGs. The absolute abundance of aminoglycoside resistance genes (AmRGs), sulfonamide resistance genes (SRGs), macrolide resistance genes (MRGs), and multidrug efflux-associated ARGs detected after the UV/Cl2 process was relatively low, and this process outperformed the others in removing a greater number of ARGs. Additionally, certain ARGs and bacterial genera were found to be enriched after the combined processes, with lower and more similar abundance levels of ARGs and genera observed after UV/Cl2 and UV-Cl2 processes compared to the Cl2-UV process. Health risk assessments indicated that the Cl2-UV process posed the highest risk, followed by UV/Cl2 and UV-Cl2 processes. Overall, the UV/Cl2 process may offer the most practical advantages for controlling antibiotic resistance.
More Related Videos
11:15Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations
Published on: July 24, 2021
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Related Concept Videos
Antibiotic Selection
Development of Antibiotic Resistance
Combined Effects of Drugs: Synergism
Such synergistic combinations...
Gene Regulation in Microbial Communities: Quorum Sensing
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Mutations
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...