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Inhibition of microRNA-660-5p decreases breast cancer progression through direct targeting of TMEM41B

Valeria Villarreal-García1, José Roberto Estupiñan-Jiménez1, Vianey Gonzalez-Villasana2

  • 1Facultad de Ciencias Biológicas, Departamento de Biología Celular y Genética, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.

Hereditas
|December 21, 2024
PubMed
Abstract

Insights

MicroRNA-660-5p (miR-660-5p) is upregulated in breast cancer, promoting cell proliferation, migration, invasion, and angiogenesis. Inhibiting miR-660-5p and targeting its effector TMEM41B may offer novel therapeutic strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading cause of cancer death in women, often driven by metastasis and drug resistance.
  • Dysregulated microRNAs (miRNAs) are implicated in breast cancer progression, metastasis, and drug resistance.
  • miR-660-5p is overexpressed in breast tumors, but its downstream targets and functions remain unclear.

Purpose of the Study:

  • To investigate the role of miR-660-5p in breast cancer cell proliferation, migration, invasion, and angiogenesis.
  • To identify downstream targets of miR-660-5p in breast cancer cells.

Main Methods:

  • Quantitative real-time PCR to measure miR-660-5p expression.
  • Functional assays (proliferation, migration, invasion) in breast cancer cell lines.
  • Angiogenesis assays using HUVEC cells.
  • Bioinformatics analysis to predict miRNA targets.
  • Western blot and dual-luciferase reporter assays for target validation.

Main Results:

  • miR-660-5p was significantly upregulated in breast cancer cell lines (MDA-MB-231, MCF-7) compared to normal cells (MCF-10A).
  • Inhibition of miR-660-5p reduced breast cancer cell proliferation, migration, invasion, and angiogenesis.
  • Bioinformatics identified 15 potential miR-660-5p targets; TMEM41B was validated as a direct target.

Conclusions:

  • miR-660-5p is upregulated and contributes to breast cancer progression, including proliferation, migration, invasion, and angiogenesis.
  • TMEM41B is a direct downstream target of miR-660-5p in breast cancer.
Keywords:
Breast CancerCancer ProgressionMicroRNAsTransmembrane Protein 41BmiR-660-5p

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