Photoactive broad-spectrum dressings with antimicrobial and antitumoral properties

In this work the development of photoactive dressings (PAD) with dual purpose, is presented. These PAD can be used for the topical treatment of persistent infections caused by fungi and bacteria and are also applicable in light antitumor therapy for carcinoma. The synthesized PAD were designed employing conjugated polymer nanoparticles (CPN) doped with platinum porphyrin which serve as polymerization photoinitiators and photosensitizers for the production of reactive oxygen species (ROS). This approach led to the synthesis of N-vinyl-2-pyrrolidone (NVP) hydrogels co-polymerized with [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC). NVP and METAC were selected to impart a good biocompatibility with eukaryotic cell lines and antimicrobial properties, respectively. The combination of METAC with an efficient photogeneration of ROS by doped CPN resulted in a material with outstanding antimicrobial features. These dressings are capable of producing an aseptic environment upon irradiation and demonstrates a bacteriostatic profile in dark conditions. Additionally, the dressings fulfill critical requirements for topical applications, providing protection and acting as a barrier, with appropriate mechanical and swelling properties; as well as adequate water vapor transmission rates. The synthesized PAD have been shown to be biocompatible and non-toxic to erythrocytes and HaCaT cell line. PAD demonstrated efficacy in eliminating microbes such as fungi and bacteria. The underlying light-induced killing mechanism involved protein photooxidation, which amplified the effects of METAC mechanism that disrupt cellular membranes. Furthermore, in vitro studies using carcinoma cell lines displayed a complete cell eradication using a relatively low light dose (36 J/cm² at 395 nm). These promising results reveal also the potential of PAD in the treatment of skin cancer.