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Updated: Jun 4, 2025

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Design, synthesis, and molecular docking of 4‑Thiol‑N‑acetylhexosamine uridine diphosphate donors for glycan
Jie Zheng1, Yang Cao1, Qiang Tao1
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China.
Abstract:
Unnatural chemically modified nucleotide sugars UDP-4-SH-GalNAc was synthesized for the first time from a previously reported Benzyl 2-Amino-glucoypranoside derivative, with a total yield of 18.6%. UDP-4-SH-GalNAc was smaller in size than UDP-GalNAc calculated by Multiwfn, and its binding capability with glycosyltransferase was stronger than that of UDP-GalNAc and glycosyltransferase, which means the unnatural nucleotide sugars is equally well recognized by the enzyme and doped into the sugar chains. These findings support the utilization of glycosyltransferases for labeling of target glycans with sulfhydryl bioorthogonal reaction groups from UDP-4-SH-GalNAc, and synthesizing glycan chains with more stable thioside bonds.
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