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Updated: Jun 4, 2025

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
Published on: July 28, 2023
Generation of induced pluripotent stem cell line, NCHi027-A, from a female child with Posterior Cloaca (Type B)
Cintia E Gomez Limia1, Karunya Albert1, Chaitrali Atre1
1Kidney and Urinary Tract Center, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA.
Abstract:
Cloaca is an ultra-rare severe anorectal malformation in females where the gastrointestinal, genital, and urologic systems converge. Posterior Cloaca (Type B) is an extremely rare specific variant, where the urogenital sinus opens just anterior to the anus. NCHi027-A is an iPSC line derived from skin fibroblasts of a 4 year and 8-month-old female with Posterior Cloaca (Type B) using Sendai Virus reprogramming. This iPSC line shows typical iPSC morphology, has normal karyotype, expresses pluripotency markers, and can be differentiated into three germ layers.

