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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Fluorescent biosensor for ultra-stability detection of Pax-5a based on a double cascade amplification strategy.

Chang Liu¹, Xinyi Zhang¹, Jun Xu¹

¹Key Laboratory of Energy Catalysis and Conversion of Nanchang, College of Chemistry and Materials, Jiangxi Normal University, Nanchang 330022, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|December 22, 2024

Summary

This study introduces a novel dual amplification method for detecting the Pax-5a gene, crucial in B-lymphoblastic leukemia development. The biosensor offers high sensitivity and specificity for early clinical diagnosis.

Keywords:

Acute lymphoblastic leukemia Double cascade amplification Entropy-driven toehold-mediated strand displacement Fluorescent biosensor Ultra-stability

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Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

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Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Aberrant expression of Pax-5a is closely linked to the development of B-lymphoblastic leukemia.
Accurate and sensitive detection of Pax-5a is vital for early diagnosis and treatment of this leukemia.

Purpose of the Study:

To develop a novel, highly sensitive, and specific dual signal amplification strategy for detecting the Pax-5a gene.
To combine Rolling Circle Amplification (RCA) and Entropy-driven Toehold-Mediated Strand Displacement (ETSD) for enhanced detection.

Main Methods:

A dual signal amplification strategy integrating RCA and ETSD was employed for Pax-5a detection.
The method utilizes sequence-specific hybridization for high specificity.
A fluorescent biosensor format was developed for signal readout.

Main Results:

The method achieved a reliable linear correlation for Pax-5a detection within a specific range.
A low detection limit of 3.34 pM was obtained.
Exceptional sensitivity was demonstrated, with recovery rates between 96.68-101.76% and a relative standard deviation of 5.47% in 1% human serum.

Conclusions:

The developed dual amplification biosensor provides a sensitive, specific, and stable method for Pax-5a detection.
This novel approach offers a promising new tool for the early clinical diagnosis of acute lymphoblastic leukemia.