Exosome laden sprayable thermo-sensitive polysaccharide-based hydrogel for enhanced burn wound healing

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Wound Healing Research

Background:

• Severe burns cause pain, inflammation, and infection, complicating recovery.
• Existing exosome hydrogels lack adaptability and sufficient antibacterial properties for burn wounds.

Purpose of the Study:

• To develop a novel, sprayable, thermosensitive hydrogel for enhanced burn wound treatment.
• To evaluate the therapeutic efficacy of exosome-loaded hydrogel (ADA-aPF127@LL18/Exo) in a deep partial thickness burn model.

Main Methods:

• Fabrication of alginate dialdehyde (ADA) and aminated Pluronic F127 (aPF127) hydrogel via Schiff base reaction.
• Incorporation of exosomes into the hydrogel for sustained release and antibacterial activity.
• Assessment of biocompatibility, cell proliferation, migration, and in vivo efficacy in a burn model.

Main Results:

• The novel hydrogel (ADA-aPF127@LL18/Exo) demonstrated sustained exosome release and enhanced antibacterial properties.
• In vitro studies showed excellent biocompatibility, promoting cell proliferation and migration.
• In vivo burn model showed accelerated epithelialization, granulation tissue formation, collagen deposition, hair follicle regeneration, reduced inflammation, and increased neovascularization.

Conclusions:

• ADA-aPF127@LL18/Exo is a promising therapeutic dressing for deep burn treatment.
• The hydrogel's multifaceted properties support efficient wound management and tissue regeneration.