Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

87.3K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.3K
RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative in silico analysis identifies functionally and regulatively relevant nsSNPs in the TRIB3 gene.

Computational biology and chemistry·2026
Same author

Silymarin-loaded phytosomal nanoparticles mitigate dyslipidemia, oxidative damage, and hepatic injury in high-fat diet-induced metabolic dysfunction-associated steatotic liver disease in rats.

BMC gastroenterology·2026
Same author

Serum-derived albumin as a cryoprotective agent for the long-term storage of red blood cells: A preliminary examination.

Transfusion·2026
Same author

Enhancing voluntary blood donations: King Fahad Armed Forces Hospital experience with mobile donation campaign.

Frontiers in public health·2026
Same author

Implications of occult hepatitis B infection for blood donor management: a five-year evaluation from Western Saudi Arabia.

BMC infectious diseases·2026
Same author

Donor retention and return patterns in Saudi Arabia: Implications for blood safety and supply stability.

Transfusion medicine (Oxford, England)·2025

Related Experiment Video

Updated: Jun 4, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.1K

RHCE genotyping using next generation sequencing: Allele specific reference sequences.

Wajnat A Tounsi1,2, Amr J Halawani3, Kelly A Sillence2,4

  • 1Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

Transfusion
|December 22, 2024
PubMed
Summary
This summary is machine-generated.

This study established new reference sequences for RHCE alleles using next-generation sequencing. Intronic variants offer a novel diagnostic approach for Rh gene variants and haplotype prediction.

Keywords:
RHCE genotypingRHCE*Ce alleleRHCE*cE alleleRHCE*ce alleleRh blood group systemion personal genome machinenext generation sequencingreference sequences

More Related Videos

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.3K
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.2K

Related Experiment Videos

Last Updated: Jun 4, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.1K
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.3K
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.2K

Area of Science:

  • Genetics
  • Molecular Biology
  • Immunology

Background:

  • The Rh blood group system (ISBT004) involves RHD and RHCE genes, producing 56 immunogenic antigens.
  • Polymorphisms in RHD and RHCE genes are clinically significant in transfusion medicine.

Purpose of the Study:

  • To establish RHD allele-specific reference sequences by sequencing the complete RHCE gene.
  • To identify and characterize novel variants within the RHCE gene.

Main Methods:

  • Sequencing of the complete RHCE gene using Next-Generation Sequencing (NGS) with the Ion Personal Genome Machine (Ion PGM).
  • Amplification of the RHCE gene via overlapping long-range PCR amplicons from 87 genomic DNA samples.
  • Analysis of sequence data against human genome reference build hg38 to identify single nucleotide variants (SNVs).

Main Results:

  • Detection of exonic SNVs encoding known RHCE variant alleles (e.g., RHCE*Ce.09, RHCE*ceAR, RHCE*ceVS.03).
  • Identification of numerous intronic SNVs linked to specific Rh haplotypes.
  • Establishment and GenBank submission of three new RHCE reference sequences (for RHCE*Ce, RHCE*cE, and RHCE*ce alleles).

Conclusions:

  • Intronic SNVs represent a potential novel diagnostic method for RH gene variants.
  • Intronic SNVs may aid in predicting Rh haplotypes.
  • This research enhances the understanding of RHCE gene polymorphism and its clinical implications.