Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.2K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ProteoMeter: a pipeline for integrating multi-PTM and limited proteolysis data to reveal modification-structure coupling at the residue level.

NAR genomics and bioinformatics·2026
Same author

Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

Clinical chemistry·2026
Same author

Phosphoproteomics in Vascular Biology and Disease: Illuminating Signaling in the Vessel.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Aggregation Methods for Quantifying PTM and Structural Changes in Bottom-Up Proteomics.

Journal of proteome research·2026
Same author

Multi-Tiered µDicer Enables Protein-Preserving Microdissection at 10 µm Resolution.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Thiol post-translational modifications modulate allosteric regulation of the OpcA-G6PDH complex through conformational gate control.

Protein science : a publication of the Protein Society·2026

Related Experiment Video

Updated: Jun 4, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K

Spatial Proteomics towards cellular Resolution.

Yumi Kwon1, James M Fulcher1, Ljiljana Paša-Tolić1

  • 1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.

Expert Review of Proteomics
|December 23, 2024
PubMed
Summary

Untargeted mass spectrometry spatial proteomics offers a powerful approach to understanding molecular organization within tissues. Advancements in tissue dissection, sample processing, and LC-MS are driving spatial proteomics towards cellular resolution.

Keywords:
Laser-capture microdissectionmass spectrometrynanoPOTSproteomicssingle-cell proteomicsspatial biologyspatial omics

More Related Videos

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.1K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

9.9K

Related Experiment Videos

Last Updated: Jun 4, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.1K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

9.9K

Area of Science:

  • Spatial biology and omics technologies
  • Molecular profiling of tissue microenvironments

Background:

  • Spatial biology leverages spatial omics for biological discovery.
  • Technologies like spatial transcriptomics, genomics, immunofluorescence, and metabolomics enable high-resolution spatial profiling.
  • These advancements deepen understanding of molecular organization in tissue microenvironments.

Purpose of the Study:

  • To provide an overview of untargeted, bottom-up mass spectrometry (MS)-based spatial proteomics.
  • To highlight recent progress in key workflow components.
  • To discuss future directions for advancing spatial proteomics.

Main Methods:

  • Overview of untargeted, bottom-up mass spectrometry (MS)-based spatial proteomics workflow.
  • Discussion of tissue dissection techniques.
  • Review of sample processing and liquid chromatography (LC)-MS technologies.

Main Results:

  • Recent progress in tissue dissection, sample processing, and LC-MS technologies is advancing spatial proteomics.
  • Current workflows are moving spatial proteomics towards cellular resolution.

Conclusions:

  • Untargeted MS-based spatial proteomics is a rapidly evolving field with significant promise.
  • Further advancements in data analysis, automated tissue dissection, and high-throughput LC-MS are critical.
  • Significant progress in dissection technologies, instrumentation, and computational tools is needed to realize the full potential.