Related Experiment Video
Updated: Jun 4, 2025
10:18
Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates
Published on: July 9, 2020
2.9K
Deep two-photon voltage imaging with adaptive excitation.
Shitong Zhao1, Eric Hebert1, Anna Gruzdeva2
1School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
Research Square
|December 23, 2024
Summary
High-speed deep brain imaging of neuronal voltage dynamics is now possible using adaptive excitation. This technique illuminates only regions of interest, enabling clearer visualization of neural activity in awake mice.
Area of Science:
- Neuroscience
- Optical Imaging
- Biophysics
Background:
- Optical imaging of neuronal voltage dynamics is crucial for understanding brain function.
- Current methods face limitations in high-speed, deep-tissue imaging due to excitation power and pixel dwell time constraints.
Purpose of the Study:
- To develop and demonstrate a high-speed, deep voltage imaging technique for neuronal activity.
- To overcome the limitations of conventional optical imaging in biological tissues.
Main Methods:
- Implementation of adaptive excitation to selectively illuminate regions of interest.
- Application of the technique to two-photon microscopy for neuronal voltage imaging.
Main Results:
- Achieved high-speed voltage imaging at depths of 500-630 μm in the awake mouse brain.
- Demonstrated successful imaging of neuronal voltage activities across two planes.
- The adaptive excitation method overcomes typical imaging depth and speed limitations.
Conclusions:
- Adaptive excitation enables high-speed, deep optical imaging of neuronal voltage dynamics.
- The technique is compatible with standard two-photon microscopes, offering broad applicability.
- This advancement facilitates deeper and faster investigation of neural circuits in vivo.
