Enhanced Electrochemiluminescence Detection of Dopamine Using Antifouling PEDOT-Modified SPEs for Complex Biological...

Area of Science:

• Biomedical analysis
• Electrochemistry
• Biosensing

Background:

• Detecting biomarkers in complex biological samples without pretreatment is challenging.
• Electrochemical methods, particularly electrochemiluminescence (ECL), offer high sensitivity and low background noise.

Purpose of the Study:

• To investigate the ECL performance of screen-printed electrodes (SPEs) modified with poly(3,4-ethylenedioxythiophene) (PEDOT) and its derivatives for dopamine (DA) detection.
• To enhance the sensitivity, stability, and specificity of DA detection in complex biological matrices.

Main Methods:

• Modification of screen-printed electrodes (SPEs) with PEDOT and its derivatives, including PEDOT-EG4-OMe.
• Electrochemical analysis using electrochemiluminescence (ECL) for dopamine detection.
• Testing sensor performance in solutions with high protein concentrations and artificial cerebrospinal fluid.

Main Results:

• PEDOT modification significantly enhanced ECL intensity, sensitivity, and linear range for DA detection.
• Functionalization with ethylene glycol (EG) improved stability, specificity, and interference resistance.
• The PEDOT-EG4-OMe modified SPEs achieved a linear range of 1-200 μM and a detection limit of 0.887 nM.
• Reliable DA detection was demonstrated in complex biological samples like high-protein solutions and artificial cerebrospinal fluid.

Conclusions:

• PEDOT derivative-modified SPEs provide a sensitive, reusable platform for dopamine detection in challenging biological environments.
• The developed ECL system shows significant potential for various demanding biomedical applications.
• This approach overcomes limitations of traditional biomarker detection methods.