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Colorimetric Hybridization Sensor for DNA Mimic of a SARS-CoV-2 RNA Marker: Direct and Inverse Bioanalysis.

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A novel colorimetric biosensor detects SARS-CoV-2 DNA mimics in undiluted biofluids without instrumentation. This practical sensor achieves low detection limits, enabling point-of-need diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Traditional biosensors often require sample dilution and instrumentation, limiting point-of-need applications.
  • Developing rapid, sensitive, and user-friendly diagnostic tools for infectious diseases is crucial.

Purpose of the Study:

  • To develop and validate a colorimetric visual biosensor for direct detection of SARS-CoV-2 RNA mimics in undiluted biofluids.
  • To assess the biosensor's performance in various matrices, including buffer, serum, and saliva.

Main Methods:

  • Utilized magnetic nanoparticles (Fe3O4) functionalized with oligonucleotide probes for target capture via hybridization.
  • Employed a secondary biotin-streptavidin-peroxidase detection system for visual colorimetric readout.
  • Performed assays in buffer, undiluted serum, and undiluted saliva to evaluate matrix effects.

Main Results:

  • Achieved detection limits as low as 1 fM in buffer and 1 pM in undiluted serum and saliva.
  • Established linear ranges from 1 fM to 10 nM (buffer) and 1 pM to 1 nM (biofluids).
  • Demonstrated the biosensor's analytical performance and sensitivity in complex biological samples.

Conclusions:

  • The developed colorimetric biosensor is practical for point-of-need diagnostics due to its direct application in undiluted biofluids and lack of instrumentation.
  • The sensor shows high sensitivity and specificity, making it a promising tool for rapid disease detection.
  • Further studies can explore its application for detecting actual viral RNA in clinical samples.