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Pooled Nanoparticle Screening Using a Chemical Barcoding Approach.

Katherine Vaidya1, Michael S Regan2, James Lin1

  • 1Department of Chemical Engineering and Materials Science, University of Minnesota Twin Cities, Minneapolis, MN, USA.

Angewandte Chemie (International Ed. in English)
|December 23, 2024
PubMed
Summary
This summary is machine-generated.

We developed a novel small molecule barcoding platform for tracking nanoparticle delivery. This method enables high-sensitivity screening of nanoparticle uptake in cancer cells without altering their properties.

Keywords:
barcodinggas chromatographymass spectrometrynanoparticlespooled screening

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Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Screening nanoparticle delivery is crucial for developing effective nanomedicines.
  • Current methods for tracking nanoparticles can be limited in sensitivity or may alter nanoparticle properties.

Purpose of the Study:

  • To develop a sensitive and non-perturbing barcoding platform for pooled screening of nanoparticle delivery.
  • To demonstrate the utility of this platform for quantifying nanoparticle uptake in cancer cells.

Main Methods:

  • Development of aryl halide-based tags (halocodes) for small molecule barcoding.
  • High-sensitivity detection of halocodes using gas chromatography-mass spectrometry (GC-MS) or electron capture detection.
  • Synthesis of a halocoded library of polylactide-co-glycolide (PLGA) nanoparticles for pooled screening.
  • Quantification of nanoparticle uptake in ovarian cancer cells and spatial mapping using mass spectrometry imaging (MSI).

Main Results:

  • Achieved high-sensitivity detection of nanoparticles at minimal halocode concentrations.
  • Demonstrated that halocoding does not alter nanoparticle physicochemical properties.
  • Quantified nanoparticle uptake in ovarian cancer cells in a pooled manner, with results correlating to conventional assays.
  • Showcased the potential of halocodes for spatial mapping of nanoparticles.

Conclusions:

  • Halocoding offers an accessible and modular platform for pooled screening of nanoparticle delivery systems.
  • This technology enables sensitive quantification and tracking of nanocarriers in various biological settings.
  • The platform has potential applications in nanomedicine development and diagnostics.