[Study of 2,6-di(propan-2-yl)phenol detection features and localization nature in warm-blooded after intragastric...

Study of 2.6-di(propan-2-yl)phenol (2.6-di(P-2-yl)F) distribution nature in warm-blooded in case of fatal poisoning due to intragastric administration of the substance.

The warm-blooded model consisted of Wistar male rats aged about 4 months, administered 1.800 mg 2.6-di(P-2-yl)F per 1 kg of animal body weight (≈3 LD₅₀). After the rats died, 2.6-di(P-2-yl)F was isolated from their corpses' biomatrices by infusing an ethyl acetate-acetone mixture (7:3), purified by extraction (distribution in the systems trichloromethane-aqueous solution (pH 11.0-11.5) and aqueous solution (pH 2-4) - ethyl acetate), and normal pressure chromatography in a silica gel column L 40×100 µm (eluent hexane-acetone (7:3). The analyte was identified using gas chromatography with mass spectrometry (capillary (0.2 mm) column, stationary phase - polysiloxane with a ratio (in %) of methyl and phenyl substituents of 95:5), thin layer chromatography (Sorbfil plates, dynamic phase hexane-diethyl ether (9:1)), and electronic spectrophotometry (solvent - ethanol). The analyte content was quantified by spectrophotometry.

In the study, 2.6-di(P-2-yl)F mainly accumulated in the stomach contents (378.94±41.51) and tissues (175.20±17.12). Among other biomatrices obtained from poisoned rat corpses, a significant amount of the investigated toxic compound was found in the heart (142.79±13.08) and spleen (117.83±16.23), a bit smaller amounts - in the lungs (112.32±29.54) and small intestine (97.10±11.66).

A scheme for determining 2.6-di(propan-2-yl)phenol in biomaterial has been developed and applied to study the analyte localization in the body of animals poisoned by it. The highest amount of the toxicant (mg/100 g) was found in the stomach contents (378.94±41.51), its tissues (175.20±17.12), heart (142.79±13.08) and spleen (117.83±16.23).