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Updated: Jun 4, 2025
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Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Published on: November 30, 2018
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DNA Ligase I Circularises Potato Spindle Tuber Viroid RNA in a Biomolecular Condensate
Yunhan Wang1, Junfei Ma2, Jie Hao2
1Plant Molecular and Cell Biology Program, University of Florida, Gainesville, Florida, USA.
Molecular Plant Pathology
|December 23, 2024
Summary
Viroids are plant pathogens that replicate in the nucleus. This study reveals potato spindle tuber viroid RNA and DNA ligase I form a biomolecular condensate near the nucleolus for RNA processing.
Area of Science:
- Molecular Biology
- Plant Pathology
- Virology
Background:
- Viroids are small, circular, noncoding RNA pathogens infecting plants.
- Nuclear replication involves host RNA polymerase II and protein TFIIIA-7ZF.
- Differential localization of RNA strands and the ligation step remain unclear.
Purpose of the Study:
- To investigate the subcellular localization and function of DNA ligase I (LIG1) during viroid replication.
- To elucidate the mechanism of circular RNA progeny formation.
- To understand the role of biomolecular condensates in subviral pathogen infection.
Main Methods:
- Confocal microscopy to observe colocalization of LIG1 and PSTVd RNA in Nicotiana benthamiana protoplasts.
- Analysis of RNA condensation and its association with LIG1.
- Investigating the role of biomolecular condensates in viroid RNA processing.
Main Results:
- Potato spindle tuber viroid (PSTVd) RNA and LIG1 colocalize near the nucleolar region.
- This colocalized region represents a condensed state of PSTVd RNA.
- Evidence suggests PSTVd RNA and LIG1 form a biomolecular condensate for RNA processing.
Conclusions:
- Viroid RNA processing and ligation occur within a biomolecular condensate near the nucleolus.
- This finding expands the known functions of biomolecular condensates to include subviral pathogen interactions.
- Understanding viroid biogenesis aids in studying newly identified viroid-like RNAs.
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