Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

1.4K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
1.4K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.0K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.0K
Chromatographic Resolution01:15

Chromatographic Resolution

420
In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
420
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.2K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of Azido Energetic Compounds Based on 2-(Pyrazol-4-yl)pyrazolo[4,3-<i>e</i>][1,2,4]triazolo[1,5-<i>c</i>][1,2,3]triazine via an Atom-Economical Intramolecular Dutt-Wormall-Type Process.

Organic letters·2026
Same author

The gut microbiota-bile acid axis in liver transplantation: implications for postoperative complications and therapeutic strategies.

Frontiers in microbiology·2026
Same author

Skeletal-Isomeric Scaffold Design Enables a Dipyrazole[3,4-<i>b</i>;4',3'-<i>e</i>]pyrazine Platform for High-Density Energetic Materials.

Organic letters·2026
Same author

Stability-Based Proteomic Methods Add Value to Activity-Based Protein Profiling Studies.

ACS chemical biology·2026
Same author

Luoshi Neiyi prescription mitigates endometriosis by modulating nuclear receptor subfamily 5 group A member 1 methylation.

International journal of biological macromolecules·2026
Same author

Complexation mechanism of 12-crown-4-ether-calix[4]arene with Li<sup>+</sup>: toward efficient recycling of end-of-life lithium-ion batteries.

Journal of molecular modeling·2026

Related Experiment Video

Updated: Jun 4, 2025

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA
11:09

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA

Published on: January 2, 2015

14.0K

DMRIntTk: Integrating different DMR sets based on density peak clustering.

Wenjin Zhang1, Wenlong Jie2, Wanxin Cui2

  • 1Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, China.

Plos One
|December 23, 2024
PubMed
Summary
This summary is machine-generated.

Researchers can now integrate multiple differentially methylated region (DMR) sets using DMRIntTk. This toolkit enhances reliability and comprehensiveness for DNA methylation analysis, improving downstream biological insights.

More Related Videos

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
12:27

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Published on: February 15, 2017

6.9K
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

13.8K

Related Experiment Videos

Last Updated: Jun 4, 2025

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA
11:09

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment Kartchner Caverns, AZ, USA

Published on: January 2, 2015

14.0K
Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
12:27

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Published on: February 15, 2017

6.9K
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

13.8K

Area of Science:

  • Genomics
  • Epigenetics
  • Bioinformatics

Background:

  • Identifying differentially methylated regions (DMRs) is crucial in DNA methylation analysis.
  • Discrepancies arise from varied prediction methods, challenging the selection of reliable DMR sets.

Purpose of the Study:

  • To develop DMRIntTk, a novel toolkit for integrating multiple DMR sets predicted from the same dataset.
  • To enhance the reliability and comprehensiveness of DMR identification for downstream analyses.

Main Methods:

  • Genome segmentation into bins and evaluation of DMR set reliability at varying methylation thresholds.
  • Bin weighting based on overlapping DMR sets and integration using a density peak clustering algorithm.
  • Application of DMRIntTk across diverse scenarios, including subtle and large methylation differences.

Main Results:

  • DMRIntTk effectively filters out regions with minor methylation differences, increasing the proportion of significant DMRs.
  • Integrated DMR sets demonstrate high comprehensiveness, with functional analyses revealing enrichment in disease-associated pathways.
  • Comparative analysis confirms the superiority of DMRIntTk over individual DMR sets, providing unique biological insights.

Conclusions:

  • DMRIntTk provides a robust solution for researchers to obtain reliable and comprehensive DMR sets.
  • The toolkit aids in overcoming challenges associated with integrating predictions from multiple DMR identification methods.