Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Batteries and Fuel Cells03:12

Batteries and Fuel Cells

27.0K
A battery is a galvanic cell that is used as a source of electrical power for specific applications. Modern batteries exist in a multitude of forms to accommodate various applications, from tiny button batteries such as those that power wristwatches to the very large batteries used to supply backup energy to municipal power grids. Some batteries are designed for single-use applications and cannot be recharged (primary cells), while others are based on conveniently reversible cell reactions that...
27.0K
Electrolysis03:00

Electrolysis

26.0K
In a galvanic cell, the electrical work is done by a redox system on its surroundings as electrons produced by the spontaneous redox reactions are transferred through an external circuit. Alternatively, an external circuit does work on a redox system by imposing a voltage sufficient to drive an otherwise nonspontaneous reaction in a process known as electrolysis. For instance, recharging a battery involves the use of an external power source to drive the spontaneous (discharge) cell reaction in...
26.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptome mining and comparative genomics reveal 36 putative novel marafivirus species and conserved evolution of the marafibox regulatory element.

Genes & genomics·2026
Same author

Charge characteristics of fluorescent proteins modulate FUS LCD condensation.

Biophysical journal·2026
Same author

A CRISPR interference system for tunable gene expression integrated with a promoter library for <i>Eubacterium callanderi</i> KIST612, an acetogen of functional diversity and versatility.

Microbiology spectrum·2026
Same author

Phantom Brain model Replicating Multiple ECoG Signals for Preclinical Device Testing.

IEEE transactions on bio-medical engineering·2026
Same author

Orexin-A Inhibits Lipopolysaccharide-Induced Cell Migration in Cultured Mouse Astrocytes via Activation of Orexin 1 Receptor: Involvement of GABA Signaling.

Journal of cellular biochemistry·2026
Same author

Merkel cells release ATP in response to mechanical stimulation to mediate paracrine signalling.

The British journal of dermatology·2026

Related Experiment Video

Updated: Jun 4, 2025

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

10.0K

A Redox-Enzyme Integrated Microbial Fuel Cell Design Using the Surface Display System in Shewanella oneidensis MR-1.

Seungwoo Baek1, Hyeryeong Lee2, Yoo Seok Lee3

  • 1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.

ACS Applied Materials & Interfaces
|December 23, 2024
PubMed
Summary
This summary is machine-generated.

This study engineered Shewanella oneidensis MR-1 to display a redox enzyme, significantly boosting its performance in a redox-enzyme integrated microbial fuel cell (REI-MFC). The enhanced exoelectrogen activity improved current generation and reduced charge transfer resistance.

Keywords:
Shewanella oneidensis MR-1autotransporterbacterial cell surface displayenzymatic biofuel cellmicrobial fuel cellredox-enzyme integrated microbial fuel cell (REI-MFC)

More Related Videos

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
05:29

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

7.6K
Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
11:58

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Published on: December 29, 2013

13.3K

Related Experiment Videos

Last Updated: Jun 4, 2025

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

10.0K
Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
05:29

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

7.6K
Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
11:58

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Published on: December 29, 2013

13.3K

Area of Science:

  • Biotechnology
  • Electrochemistry
  • Microbial Fuel Cells

Background:

  • Biofuel cells utilize exoelectrogens or biocatalysts for electron transfer.
  • Genetic engineering enhances microbial exoelectrogen activity for improved biofuel cell performance.

Purpose of the Study:

  • To demonstrate a redox-enzyme integrated microbial fuel cell (REI-MFC) design.
  • To enhance the electrogenic activity of Shewanella oneidensis MR-1 by displaying an additional redox enzyme on its cell surface.

Main Methods:

  • Constructed a cell-surface display system for Shewanella oneidensis MR-1 using an Escherichia coli autotransporter.
  • Engineered MR-1 to display swine NADH-cytochrome b5 reductase 3 (B5R3).
  • Validated the display system's functionality and tested the engineered strain in an MFC environment.

Main Results:

  • The engineered MR-1 strain displaying B5R3 showed a 4.7-fold increase in current generation compared to wild-type MR-1 (10.4 ± 1.9 μA vs. 2.2 ± 0.3 μA).
  • Maximum charge transfer resistance was reduced by 70% in the engineered strain.
  • The cell surface display system effectively facilitated exoelectrogen activity in the REI-MFC.

Conclusions:

  • The developed cell-surface display system enhances exoelectrogen activity in Shewanella oneidensis MR-1.
  • This strategy significantly improves the performance of redox-enzyme integrated microbial fuel cells.
  • The findings offer a promising approach for advancing biofuel cell technology.