Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

3.9K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
3.9K
Microbial Fermentation01:23

Microbial Fermentation

1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
Amino Acid Catabolism01:18

Amino Acid Catabolism

1.7K
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
1.7K
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

873
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
873
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

928
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
928
Production of Organic Acids01:25

Production of Organic Acids

105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chassis Selection and Metabolic Fine-Tuning Enable Efficient in planta Betalain Production.

Plant physiology·2026
Same author

Environmental stress reveals new insights regarding proteome rebalancing in Arabidopsis thaliana seeds.

The Plant journal : for cell and molecular biology·2026
Same author

Designing a nitrogen-efficient cold-tolerant maize for modern agricultural systems.

The Plant cell·2025
Same author

Grass Rhizome Proteomics Reveals Convergent Freezing-Tolerance Strategies.

bioRxiv : the preprint server for biology·2025
Same author

Multiple methods for assessing learning and memory in <i>Drosophila melanogaster</i> demonstrates the highly complex, context-dependent genetic underpinnings of cognitive traits.

bioRxiv : the preprint server for biology·2025
Same author

Amino Acid Quantification from Maize Tissues.

Cold Spring Harbor protocols·2024
Same journal

Emerging Trends in Mass Spectrometry-Based Quantitative Proteome and Phosphoproteome Profiling in Maize.

Cold Spring Harbor protocols·2026
Same journal

Sample Preparation for Quantitative Proteome and Phosphoproteome Profiling of Maize Tissues.

Cold Spring Harbor protocols·2026
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: May 1, 2026

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.
05:55

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.

Published on: June 16, 2018

6.9K

High-Throughput Free Amino Acid Quantification from Maize Tissues.

Huda Ansaf1, Abou Yobi1, Ruthie Angelovici2

  • 1Christopher S. Bond Life Sciences Center, Division of Biological Sciences, Interdisciplinary Plant Group, University of Missouri, Columbia, Missouri 65211, USA.

Cold Spring Harbor Protocols
|December 23, 2024
PubMed
Summary
This summary is machine-generated.

This study presents a new method for quantifying free amino acids (FAAs) in maize. The robust protocol accurately measures all 20 proteogenic FAAs, crucial for understanding plant responses.

More Related Videos

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
10:50

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

14.6K
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the &#945;-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

10.5K

Related Experiment Videos

Last Updated: May 1, 2026

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.
05:55

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.

Published on: June 16, 2018

6.9K
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
10:50

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

14.6K
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the &#945;-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

10.5K

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Amino acids in maize exist as free or protein-bound forms.
  • Free amino acids (FAAs) are vital signaling molecules, nitrogen transporters, osmolytes, and metabolic precursors.
  • FAA levels in maize vary with development and environmental conditions, necessitating precise quantification.

Purpose of the Study:

  • To describe a robust, high-throughput method for quantifying free amino acids in maize tissue.
  • To enable accurate measurement of all 20 proteogenic amino acids in their free state.

Main Methods:

  • Aqueous extraction of FAAs from maize tissue.
  • Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for separation, detection, and quantification.
  • Data analysis using MassLynx and TargetLynx software.

Main Results:

  • A validated method for quantifying 20 proteogenic FAAs in maize.
  • High-throughput capability for analyzing free amino acid profiles.
  • Reliable quantification for studying developmental and environmental responses.

Conclusions:

  • The described method provides a reliable and efficient means to quantify FAAs in maize.
  • Accurate FAA quantification is essential for maize research on plant responses.
  • This protocol supports advancements in understanding maize physiology and metabolism.