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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Jun 4, 2025

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Bottom-Up Proteomics Workflow for Studying Multi-organism Systems.

Hongxia Bai¹, Leonard B Collins², Marcos Rogério André^3,4

¹Department of Chemistry, North Carolina State University, Raleigh, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 23, 2024

Summary

This summary is machine-generated.

This study introduces a novel proteomics workflow to analyze protein changes in fleas infected with Bartonella henselae. The method helps understand host-pathogen interactions in multi-organism research.

Keywords:

Bartonella henselae Bottom-up proteomics Ctenocephalides felis felis (flea)Differential protein expression nanoLC-MS/MS

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

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Published on: October 24, 2018

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Last Updated: Jun 4, 2025

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

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Published on: October 19, 2021

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Published on: November 15, 2017

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Area of Science:

Proteomics
Microbiology
Vector-borne diseases

Background:

Discovery proteomics is crucial for understanding complex biological interactions.
Studying host-pathogen relationships requires advanced analytical techniques.

Purpose of the Study:

To develop and detail a bottom-up proteomics workflow for analyzing differential protein expression.
To investigate protein expression changes in cat fleas (Ctenocephalides felis felis) experimentally infected with Bartonella henselae.

Main Methods:

Bottom-up proteomics workflow.
Nano liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS).
Label-free quantification and Proteome Discoverer software for data analysis.

Main Results:

Successfully identified and quantified differential protein expression in infected fleas.
Established a reproducible methodology for multi-organism proteomic studies.

Conclusions:

The developed proteomics workflow is effective for examining host-pathogen interactions.
This protocol provides a foundation for diverse multi-organism proteomic investigations.