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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
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Mass Spectrometry-Based Glycomics and Proteomics Profiling of On-Slide Digested Tissue from Complex Biological

Sayantani Chatterjee1, Joseph Zaia1,2, Manveen K Sethi3

  • 1Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 23, 2024
PubMed
Summary

This study presents an on-slide tissue digestion method for comprehensive glycoproteome mapping. This technique enables detailed profiling of glycosaminoglycans, N-linked glycans, and (glyco)peptides from small tissue samples.

Keywords:
Formalin-fixed paraffin-embedded tissueFresh frozen tissueGlycomicsGlycoproteomicsLiquid chromatographyMass spectrometryOn-slide tissue digestionProteomics

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