Related Experiment Video
Updated: Jul 10, 2026
09:09
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
Published on: May 18, 2016
13.6K
Intestinal organoid coculture systems: current approaches, challenges, and future directions.
Ghanyah Al-Qadami1, Anita Raposo2, Chia-Chi Chien3
1Health and Biosecurity, CSIRO, Adelaide, South Australia, Australia.
American Journal of Physiology. Gastrointestinal and Liver Physiology
|December 23, 2024
Summary
Intestinal organoid coculture models integrate epithelial, nonepithelial, and microbial components to better mimic the gut microenvironment. This review explores methods for creating these complex models to study intestinal health and disease.
Area of Science:
- Gastroenterology and Microbiology
- Stem Cell Biology and Tissue Engineering
- Infectious Disease Modeling
Background:
- The intestinal microenvironment is a complex ecosystem of cells, mucus, and microbiota crucial for health and disease.
- Intestinal organoids, derived from adult stem cells, offer a 3-D model of the gut but lack key nonepithelial and microbial components.
- Current organoid models do not fully recapitulate the intricate interactions within the native intestinal microenvironment.
Purpose of the Study:
- To review existing methodologies for establishing intestinal organoid coculture systems.
- To highlight the strengths and limitations of various coculture approaches.
- To discuss future directions in recreating the complex intestinal microenvironment using organoid technology.
Main Methods:
- Coculturing intestinal organoids with microbes (bacteria, viruses).
- Integrating organoids with immune, stromal, and neural cells.
- Developing three-dimensional (3-D) mini-intestine models that mimic native architecture and cellular diversity.
Main Results:
- Coculture systems allow for the recreation of the complex intestinal environment.
- These models facilitate the study of intricate cross-talk between intestinal components under healthy and pathological conditions.
- Various approaches exist for establishing intestinal organoid cocultures, each with unique advantages and disadvantages.
Conclusions:
- Intestinal organoid cocultures are powerful platforms for investigating gut physiology and pathology.
- These advanced models are essential for understanding host-microbe and cell-cell interactions in the gut.
- Further development of coculture methodologies will enhance our understanding of intestinal health and disease mechanisms.
Related Concept Videos
01:17Chronic Bowel Disorders: Introduction
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
01:26Physiology of the Gastrointestinal System III: Elimination
The gastrointestinal elimination process involves a complex interplay of neural and hormonal mechanisms that coordinate the final waste removal from the body. This intricate operation encompasses the absorption of water and electrolytes, vital for transforming the remaining indigestible food matter into feces. The large intestine is pivotal in water and electrolyte absorption, forming feces from unabsorbed minerals, undigested food, bacteria, bile pigments, and shed epithelial cells. Essential...
01:29Chronic Pancreatitis II: Collaborative Care
The management of chronic pancreatitis is multifaceted, involving a comprehensive approach that includes thorough assessment, diagnostic testing, and a variety of management strategies.
Assessment:
Assessment:
01:29Intestinal Obstruction I: Introduction
Intestinal obstruction is a partial or complete blockage of the small or large intestine that disrupts the normal flow of intestinal contents through the lumen. This interruption impairs digestion, absorption, and fluid balance, and may lead to serious complications if not treated promptly.Mechanical ObstructionMechanical obstruction occurs when a physical blockage prevents intestinal contents from passing, arising from within the lumen or the bowel wall, or from external compression.Adhesions,...
01:07Intestinal Obstruction II: Pathophysiology
Intestinal obstruction triggers a series of physiological responses, starting with gas and fluid accumulation in the bowel segment proximal to the obstruction, leading to distension. This distended intestine compresses the diaphragm, hindering lung expansion and potentially leading to reduced respiratory effort, atelectasis, and pneumonia.To overcome the blockage, the gut intensifies contractions, causing colicky abdominal pain, nausea, and vomiting, which reduces fluid and food intake and...