[Evaluation of Bacterial Identification Performance Using the I-dOne Measuring Device Based on the Principle of the...

Area of Science:

• Microbiology
• Analytical Chemistry
• Spectroscopy

Background:

• Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is crucial for rapid microorganism identification, aiding antimicrobial stewardship and infectious disease diagnosis.
• The emergence of new identification technologies is vital for improving diagnostic speed and accuracy in clinical microbiology.

Purpose of the Study:

• To evaluate the performance and accuracy of the I-dOne system, an Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy-based microorganism identification system.
• To compare the identification results of the I-dOne system against the established MALDI Biotyper mass spectrometer.

Main Methods:

• A total of 174 bacterial strains (78 Gram-positive, 96 Gram-negative) were analyzed.
• Bacterial identification was performed using the I-dOne system and the MALDI Biotyper mass spectrometer.
• Identification agreement rates between the two methods were calculated.

Main Results:

• The overall identification agreement rate between the I-dOne system and MALDI Biotyper was 73.3% (255 out of 348 samples).
• Performance was evaluated across both Gram-positive and Gram-negative bacterial species.

Conclusions:

• The I-dOne system demonstrates potential for microorganism identification but requires further improvement in accuracy.
• Software updates, database expansion, and refinement are anticipated to enhance the I-dOne system's identification capabilities.