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A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration
Published on: February 27, 2018
Resveratrol ameliorates postoperative cognitive dysfunction in aged mice by regulating microglial polarization
Jinming Liu1, Yong Wang2, Hong Sun1
1Department of Anesthesiology, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu, China.
Abstract:
Postoperative cognitive dysfunction (POCD) is a common cognitive challenge faced by older adults. One of the key contributors to the development of POCD is neuroinflammation induced by microglia. Resveratrol has emerged as a promising candidate for the prevention of cognitive decline. Previous studies have demonstrated its potential in alleviating cognitive deterioration, yielding encouraging results. Nonetheless, the mechanism of resveratrol improving cognitive function remains unclear. Therefore, we assessed the effect of resveratrol in both aged POCD model mice and BV2 cells on CX3CL1/CX3CR1 axis, a critical signaling pathway mediating microglial activity. Both in vitro and in vivo experiments have revealed that pre-administration of resveratrol not only mitigates cognitive deficits but also significantly reduces the levels of inflammatory cytokines. Additionally, it enhanced the expression of SIRT1 and CX3CR1 within the hippocampal region. We also evaluated the impact of resveratrol on CX3CR1 siRNA transfected BV2 cells. Delete of CX3CR1 reversed the preventive role of resveratrol. Our findings implied that resveratrol might inhibit microglial activation and improve cognition by mediating CX3CL1/CX3CR1 signaling.
Insights
Resveratrol may prevent cognitive decline by reducing neuroinflammation. This study shows resveratrol improves cognition and reduces inflammation by affecting the CX3CL1/CX3CR1 pathway, crucial for microglial activity.
Area of Science:
- Neuroscience
- Pharmacology
Background:
- Postoperative cognitive dysfunction (POCD) is a prevalent issue in older adults, often linked to neuroinflammation mediated by microglia.
- Resveratrol shows promise in preventing cognitive decline, but its mechanism of action is not fully understood.
Purpose of the Study:
- To investigate the mechanism by which resveratrol ameliorates cognitive deficits.
- To examine the role of the CX3CL1/CX3CR1 signaling pathway in resveratrol's effects on neuroinflammation and cognition.
Main Methods:
- In vivo studies using aged POCD model mice and in vitro studies using BV2 microglial cells.
- Assessed cognitive function, inflammatory cytokine levels, and the expression of SIRT1 and CX3CR1.
- Utilized siRNA to knockdown CX3CR1 in BV2 cells to evaluate its role.
Main Results:
- Resveratrol administration mitigated cognitive deficits and reduced inflammatory cytokines in POCD mice.
- Resveratrol enhanced SIRT1 and CX3CR1 expression in the hippocampus.
- Knockdown of CX3CR1 in BV2 cells reversed the protective effects of resveratrol.
Conclusions:
- Resveratrol may inhibit microglial activation and improve cognitive function by modulating the CX3CL1/CX3CR1 signaling pathway.
- The findings suggest a potential therapeutic role for resveratrol in preventing POCD.
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