Triphenyl-Modified Mixed-Mode Stationary Phases With and Without Embedded Ion-Exchange Sites for High-Performance...

Area of Science:

• Analytical Chemistry
• Materials Science
• Chromatography

Background:

• Development of advanced stationary phases is crucial for improving separation efficiency in liquid chromatography.
• Mixed-mode chromatography offers versatile separation mechanisms by combining different interaction types.

Purpose of the Study:

• To synthesize and characterize novel triphenyl-modified silica-based stationary phases for mixed-mode liquid chromatography.
• To evaluate the chromatographic performance and potential applications of these new phases for biomolecule separations.

Main Methods:

• Synthesis of three distinct triphenyl-modified silica phases using thiol-ene and thiol-yne click reactions.
• Characterization via elemental analysis, solid-state NMR spectroscopy, and ζ-potential measurements.
• Chromatographic evaluation under reversed-phase, ion-exchange, and hydrophilic interaction modes; principal component analysis for classification.

Main Results:

• Successfully prepared three triphenyl-modified silica phases: non-ionic, strong anion-exchanger (SAX), and zwitterionic.
• Demonstrated chromatographic utility for separating biomolecules like teicoplanin and siRNA.
• Established the orthogonality of the developed stationary phases.

Conclusions:

• The novel triphenyl-modified silica phases offer distinct separation characteristics for mixed-mode liquid chromatography.
• These phases are effective for complex biomolecule separations, showcasing their potential in analytical and preparative applications.