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Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

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Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
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Hematopoiesis01:21

Hematopoiesis

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
4.9K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.0K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
2.9K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

3.6K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
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Related Experiment Video

Updated: May 8, 2025

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Deciphering hematopoietic stem cell development: key signaling pathways and mechanisms.

Saori Morino-Koga1, Tomomasa Yokomizo2

  • 1Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.

Frontiers in Cell and Developmental Biology
|December 24, 2024
PubMed
Summary

Hematopoietic stem cells (HSCs) originate from endothelial cells during development. Understanding the signals and transcription factors involved is key to generating HSCs from pluripotent stem cells.

Keywords:
aorta-gonad-mesonephros (AGM)endothelial-to-hematopoietic transition (EHT)erythro-myeloid progenitor (EMP)hematopoietic stem cell (HSC)hematopoietic stem cell precursor (pre-HSC)intra-aortic hematopoietic cluster (IAHC)

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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
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Last Updated: May 8, 2025

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
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Area of Science:

  • Developmental biology
  • Hematopoiesis
  • Stem cell biology

Background:

  • Hematopoietic stem cells (HSCs) are crucial for blood cell formation.
  • HSCs originate from endothelial cells during mid-gestation.
  • The process involves hemogenic endothelial cells transitioning to pre-HSCs and then mature HSCs.

Purpose of the Study:

  • To review the timing and roles of transcription factors in HSC induction.
  • To summarize external signals effective for in vitro HSC induction.
  • To advance methods for inducing HSCs from pluripotent stem cells.

Main Methods:

  • Literature review of transcription factors involved in HSC induction.
  • Summary of external signals demonstrated in in vitro culture systems.

Main Results:

  • Identification of key transcription factors and signaling pathways.
  • Compilation of effective in vitro culture conditions for HSC induction.

Conclusions:

  • Precise understanding of developmental signals is critical.
  • This knowledge will facilitate the generation of HSCs from pluripotent stem cells for therapeutic applications.