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Solvent interactions with test compounds and recommendations for testing to avoid artifacts.
Environmental Mutagenesis
|January 1, 1985
Summary
Solvent interactions can alter test results. This study found dimethyl sulfoxide (DMSO) reacted with hexachloroacetone, affecting mutagenicity. Researchers recommend using different solvents to confirm findings and avoid artifactual data.
Area of Science:
- Toxicology
- Chemical interactions
Background:
- Artifactual results due to test material-solvent interactions are increasingly reported.
- Previous studies indicated solvent effects with hexachloroacetone.
Purpose of the Study:
- To investigate interactions between chlorinated acetones (tri-, tetra-, pentachloro-, and hexachloroacetone) and various solvents.
- To identify potential sources of artifactual data in mutagenicity testing.
Main Methods:
- Mutagenicity testing of chlorinated acetones dissolved in acetone and dimethyl sulfoxide (DMSO).
- Analysis of time-dependent reactions between test compounds and solvents.
Main Results:
- Quantitative differences in mutagenic potency were observed for tri-, tetra-, and pentachloroacetone in acetone versus DMSO.
- Hexachloroacetone was mutagenic without solvent, with enhanced activity in DMSO, but not in acetone.
- A time-dependent reaction between hexachloroacetone and DMSO was identified, indicating DMSO is unsuitable for this compound.
Conclusions:
- Dimethyl sulfoxide (DMSO) is not an appropriate solvent for hexachloroacetone due to chemical interactions.
- Researchers recommend confirming initial screening results with a different solvent and verifying other studies' findings using alternative solvents to prevent artifactual reporting.