Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solvent interactions with test compounds and recommendations for testing to avoid artifacts.

E R Nestmann, G R Douglas, D J Kowbel

    Environmental Mutagenesis
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field.

    Radiation research·2006
    Same author

    Safety assessment and risk-benefit analysis of the use of azodicarbonamide in baby food jar closure technology: putting trace levels of semicarbazide exposure into perspective--a review.

    Food additives and contaminants·2005
    Same author

    Perspectives on the genotoxic risk of styrene.

    Journal of toxicology and environmental health. Part B, Critical reviews·2005
    Same author

    Epithelial and fibroblast cell lines cultured from the transgenic BigBlue rat: an in vitro mutagenesis assay.

    Mutation research·2001
    Same author

    Survival analysis for success of Molteno tube implants.

    The British journal of ophthalmology·2001
    Same author

    IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety.

    Mutation research·2000

    Solvent interactions can alter test results. This study found dimethyl sulfoxide (DMSO) reacted with hexachloroacetone, affecting mutagenicity. Researchers recommend using different solvents to confirm findings and avoid artifactual data.

    Area of Science:

    • Toxicology
    • Chemical interactions

    Background:

    • Artifactual results due to test material-solvent interactions are increasingly reported.
    • Previous studies indicated solvent effects with hexachloroacetone.

    Purpose of the Study:

    • To investigate interactions between chlorinated acetones (tri-, tetra-, pentachloro-, and hexachloroacetone) and various solvents.
    • To identify potential sources of artifactual data in mutagenicity testing.

    Main Methods:

    • Mutagenicity testing of chlorinated acetones dissolved in acetone and dimethyl sulfoxide (DMSO).
    • Analysis of time-dependent reactions between test compounds and solvents.

    Main Results:

    • Quantitative differences in mutagenic potency were observed for tri-, tetra-, and pentachloroacetone in acetone versus DMSO.

    Related Experiment Videos

  • Hexachloroacetone was mutagenic without solvent, with enhanced activity in DMSO, but not in acetone.
  • A time-dependent reaction between hexachloroacetone and DMSO was identified, indicating DMSO is unsuitable for this compound.

    • Conclusions:

    • Dimethyl sulfoxide (DMSO) is not an appropriate solvent for hexachloroacetone due to chemical interactions.
    • Researchers recommend confirming initial screening results with a different solvent and verifying other studies' findings using alternative solvents to prevent artifactual reporting.