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Updated: May 8, 2025
Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
Published on: January 12, 2015
Single cell transcriptomics of the cerebral cortex of mice lacking the PRC2 gene eed
Laura Currey1, Lachlan Harris2,3, Michael Piper4,5
1School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia. uqlcurre@uq.edu.au.
Objective:
The Polycomb Repressive Complex 2 (PRC2) regulates neural stem cell behaviour during development of the cerebral cortex, yet how the loss of PRC2 developmentally influences cell identity in the mature brain is poorly defined. Using a mouse model in which the PRC2 gene Embryonic ectoderm development (Eed) was conditionally deleted from the developing mouse dorsal telencephalon, we performed single nuclei RNA sequencing (snRNA-seq) on the cortical plate of an adult heterozygote Eed knockout mouse and an adult homozygote Eed knockout mouse compared to a littermate control. This work was part of a larger effort to understand consequences of mutations to PRC2 within the mature brain.
Results:
Here we provide snRNA-seq data from the cortical plate of an adult heterozygous conditional Eed knockout, an adult homozygous conditional Eed knockout and an adult control mouse. This data provides insight on how loss of PRC2 function during development affects cell identity in the mature cortex.
Insights
Loss of Polycomb Repressive Complex 2 (PRC2) during development alters mature brain cell identity. Single nuclei RNA sequencing reveals impacts of Embryonic ectoderm development (Eed) gene deletion on cortical cell types.
Area of Science:
- Neuroscience
- Developmental Biology
- Genetics
Background:
- Polycomb Repressive Complex 2 (PRC2) is crucial for regulating neural stem cell behavior during cerebral cortex development.
- The precise impact of PRC2 loss on mature brain cell identity, following developmental disruption, remains incompletely understood.
Purpose of the Study:
- To investigate the long-term effects of PRC2 deficiency on cell identity within the mature cerebral cortex.
- To analyze how conditional deletion of the Embryonic ectoderm development (Eed) gene, a key PRC2 component, influences cortical cell populations post-development.
Main Methods:
- Utilized a conditional knockout mouse model with targeted deletion of the Eed gene in the developing dorsal telencephalon.
- Performed single nuclei RNA sequencing (snRNA-seq) on the cortical plate of adult control, heterozygous Eed knockout, and homozygous Eed knockout mice.
Main Results:
- Generated snRNA-seq data characterizing the cortical plate cell composition in adult mice with varying levels of Eed deletion.
- The data provides insights into how developmental loss of PRC2 function reshapes cell identity in the mature cortex.
Conclusions:
- Developmental loss of PRC2 function leads to alterations in cell identity within the mature cerebral cortex.
- This study provides valuable molecular data for understanding the consequences of PRC2 mutations in the adult brain.
