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Morphological responses of two Aspergillus oryzae strains to various metal ions at different concentrations
Liyun Liu1, Kanae Sakai1, Takumi Tanaka1
1a Department of Biotechnology, Graduate School of Engineering, Osaka University.
Abstract:
Aspergillus species take up various metal ions from environment. The morphology of Aspergillus oryzae strains can vary under the influence of various metal ions. Here, the effects of Ti4+, V3+, Sr2+, Ba2+, Al3+, Fe2+, Zn2+, Mn2+, Ca2+, and Cu2+ on morphological parameters of A. oryzae strains RIB40 and RIB143 were estimated. Colony diameter, conidiation, vesicle head size, and stipe width in both strains varied with concentration. Ti4+, Sr2+, Ba2+, Al3+, Fe2+, and Ca2+ affected conidiation in similar tendency between two strains. The effects of Ti4+, V3+, Sr2+, and Ba2+ on the morphology of A. oryzae are reported here for the first time. Induction of growth of both strains by 0.0001% Ti4+ may help the fermentation industry. Induction of conidiation in RIB40 by 0.001% Cu2+ confirmed previous results that low concentrations of Cu2+ promote the growth of Aspergillus. The most novel finding is that 0.001% Zn2+ increased the vesicle head size in RIB40; possible reasons are discussed.
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