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Related Concept Videos

Treatment for Pulmonary Arterial Hypertension: Oxygen Therapy for Respiratory Failure01:16

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Oxygen therapy has emerged as a significant tool in enhancing the quality of life for patients suffering from pulmonary arterial hypertension (PAH). While this therapy has principally been studied on patients with significant hypoxemia, this therapeutic approach helps prevent potential organ damage and can be administered in the comfort of one's home.
Oxygen therapy is vital in increasing and maintaining blood oxygen levels in PAH patients. As a result, it aids in reducing fatigue,...
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Related Experiment Video

Updated: May 8, 2025

Non-thermal Infrared Light Treatment of Ischemia/Reperfusion Injury and Subsequent Analysis of Macrophage Differentiation
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Hyperbaric Oxygen Therapy Improved Neovascularisation Following Limb Ischaemia-The Role of ROS Mitigation.

You-Cheng Lin1, Jhih-Yuan Shih2,3,4, Yu-Wen Lin2

  • 1Division of Plastic Surgery, Department of Surgery, Chi-Mei Medical Center, Tainan, Taiwan.

Journal of Cellular and Molecular Medicine
|December 25, 2024
PubMed
Summary

Hyperbaric oxygen therapy significantly boosts blood vessel growth and reduces harmful oxidative stress in limb ischemia. This treatment enhances healing by promoting angiogenesis and suppressing reactive oxygen species (ROS).

Keywords:
HBOROSangiogenesislimb ischaemia

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Area of Science:

  • Biomedical Engineering
  • Vascular Biology
  • Oxygen Therapy

Background:

  • Limb ischemia is a condition characterized by reduced blood flow, leading to tissue damage.
  • Hyperbaric oxygen (HBO) therapy, involving exposure to high oxygen concentrations, is being explored for its potential therapeutic benefits.
  • The precise mechanisms by which HBO influences limb ischemia, particularly in hypoxic conditions, require further elucidation.

Purpose of the Study:

  • To investigate the therapeutic potential of HBO in treating limb ischemia.
  • To evaluate the effects of HBO on angiogenic capacity and oxidative stress in a mouse model of limb ischemia.
  • To explore the in vitro effects of HBO on endothelial cell function and reactive oxygen species (ROS) production.

Main Methods:

  • Wild-type C57BL/6J mice underwent limb ischemia surgery and were treated with HBO (100% oxygen at 3 ATA for 1 hour/day for five days).
  • Angiogenic capacity was assessed using laser Doppler perfusion imaging and CD31 staining.
  • In vitro experiments utilized oxygen-glucose deprivation (OGD) to induce endothelial cell dysfunction, followed by HBO treatment.

Main Results:

  • HBO treatment significantly enhanced angiogenic capability in mice with limb ischemia compared to controls.
  • HBO administration led to reduced reactive oxygen species (ROS) expression in the ischemic limbs.
  • In vitro, HBO mitigated endothelial cell dysfunction caused by OGD, rescuing impaired proliferation, migration, and tube formation.
  • HBO upregulated key proangiogenic factors including VEGF, HO-1, HIF-1, and Nrf2.

Conclusions:

  • HBO therapy demonstrates significant promise for augmenting endogenous angiogenic potential in limb ischemia.
  • HBO treatment effectively suppresses ROS levels, contributing to its therapeutic effects in limb ischemia.
  • HBO may serve as a valuable therapeutic strategy for limb ischemia by enhancing vascular repair and reducing oxidative damage.