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The immunomodulatory potential of chickpea protein hydrolysate via ROS and NO pathways
Noelia M Rodríguez-Martín1, José Carlos Márquez-López1, José Antonio González-Jurado2
1Instituto de la Grasa-CSIC, Plant Protein Group, Seville 41013, Spain.
Abstract:
The uncontrolled overproduction of Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) is linked to chronic inflammation, although they are also essential signaling molecules for the immune system against infectious agents. Bioactive compounds hold promise as functional bioactive nutrients, contributing to the immunomodulatory response. This study investigates the potential of chickpea protein hydrolysate to modulate ROS/RNS stress and inflammatory responses in a cellular low-grade chronic inflammatory model. This study was focused on their effects on endogenous antioxidant enzyme activities and key pro-inflammatory markers. ROS and nitric oxide (NO) production and molecular biology techniques were used to evaluate cell metabolism. Hydrolysate exposure notably increased ROS and NO release in a dose-dependent manner, while also exhibiting significant anti-inflammatory effects by inhibiting NF-κB and NLRP3 inflammasome components in treated cells. Therefore, chickpea protein hydrolysates hold promise as functional bioactive compounds for use in therapeutic applications, promoting human health and well-being.
Insights
Chickpea protein hydrolysate shows potential in managing inflammation by modulating reactive oxygen and nitrogen species. This bioactive compound may offer therapeutic benefits for human health.
Area of Science:
- Nutritional Science
- Immunology
- Biochemistry
Background:
- Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) are crucial for immune signaling but uncontrolled production contributes to chronic inflammation.
- Bioactive compounds, including protein hydrolysates, are investigated for their immunomodulatory and antioxidant properties.
Purpose of the Study:
- To evaluate the potential of chickpea protein hydrolysate in modulating ROS/RNS stress and inflammation.
- To assess its effects on endogenous antioxidant enzyme activities and pro-inflammatory markers in a cellular model of low-grade chronic inflammation.
Main Methods:
- Utilized molecular biology techniques to measure Reactive Oxygen Species (ROS) and nitric oxide (NO) production.
- Assessed cell metabolism and the impact of chickpea protein hydrolysate on key inflammatory pathways, including NF-κB and NLRP3 inflammasome.
Main Results:
- Chickpea protein hydrolysate exposure dose-dependently increased ROS and NO release.
- Demonstrated significant anti-inflammatory effects by inhibiting NF-κB and NLRP3 inflammasome components.
Conclusions:
- Chickpea protein hydrolysates exhibit promising functional bioactive properties for therapeutic applications.
- These hydrolysates can modulate oxidative stress and inflammatory responses, supporting human health and well-being.
